Generation of Live Fry from Intraperitoneally Transplanted Primordial Germ Cells in Rainbow Trout

Sexual plasticity of ovarian germ cells in rainbow trout.

The sexual plasticity of fish gonads declines after the sex differentiation period; however, information about the plasticity of the germ cells themselves after sex differentiation is limited. Using rainbow trout (Oncorhynchus mykiss), we recently established a novel germ cell transplantation system that provides a unique platform with which to dissect the developmental and cellular mechanisms ...

generation of in-vitro spermatogonial stem cells following genetic manipulation of primordial germ-like cells

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

Uptake of 106Ru by eggs and fry of rainbow trout.

The experimental studies on uptake and accumulation of 106Ru by artificially fertilized eggs, advanced fry and fingerlings of rainbow trout have been conducted. The accumulation in the egg previously treated with drugs for control of bacterial growth on the egg was more rapid and higher than that in non-treated egg. The major part of 106Ru in the egg was associated with the egg capsule on hatch...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

Review of Differentiation and Proliferation of Primordial Germ Cells in Culture

Primordial germ cells (PGCs) are highly specialized cell population that arises from the epiblast in vivo. There are three critical steps in the life cycle of these cells: 1-Specification 2-migration and proliferation 3-prenatal and postnatal sex specific development. Specification of germ cells in epiblast occurs due to signals secreted from extraembryonic tissues. Primordial germ cells are re...